- Abstract:
-
Three dimensional (3-D) fluorescence microscopes, including conventional instruments with digital deblurring, confocal systems and two-photon excitation, all exhibit monochromatic and chromatic aberrations. A simple Gaussian model of the aberrated point spread function and optical field for each instrument illustrates spatial distortions and blurring and some unique attenuation effects in confocal and two-photon microscopy. These properties depend on the manner in which illumination and detec...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1046/j.1365-2818.1996.113392.x
-
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- Volume:
- 181
- Issue:
- 2
- Pages:
- 99-116
- Publication date:
- 1996-02-05
- DOI:
- EISSN:
-
1365-2818
- ISSN:
-
0022-2720
- URN:
-
uuid:6aa87e83-52da-421b-80b8-0ca3489c9018
- Source identifiers:
-
45194
- Local pid:
- pubs:45194
- Copyright date:
- 1996
Conference
Aberration control in quantitative imaging of botanical specimens by multidimensional fluorescence microscopy
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