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Aberration control in quantitative imaging of botanical specimens by multidimensional fluorescence microscopy

Abstract:

Three dimensional (3-D) fluorescence microscopes, including conventional instruments with digital deblurring, confocal systems and two-photon excitation, all exhibit monochromatic and chromatic aberrations. A simple Gaussian model of the aberrated point spread function and optical field for each instrument illustrates spatial distortions and blurring and some unique attenuation effects in confocal and two-photon microscopy. These properties depend on the manner in which illumination and detec...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
Volume:
181
Issue:
2
Pages:
99-116
Host title:
JOURNAL OF MICROSCOPY-OXFORD
Publication date:
1996-02-01
DOI:
EISSN:
1365-2818
ISSN:
0022-2720
Source identifiers:
45194
Keywords:
Pubs id:
pubs:45194
UUID:
uuid:6aa87e83-52da-421b-80b8-0ca3489c9018
Local pid:
pubs:45194
Deposit date:
2012-12-19

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