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Evaluation of consensus method for the culture of Burkholderia pseudomallei in soil samples from Laos

Abstract:

Background

We have previously shown that PCR following enrichment culture is the most sensitive method to detect Burkholderia pseudomallei in environmental samples. Here we report an evaluation of the published consensus method for the culture of B. pseudomallei from Lao soil in comparison with our conventional culture method and with PCR with or without prior broth enrichment.

Methods

One hundred soil samples were collected from a field known to contain B. pseudo...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.12688/wellcomeopenres.14851.2

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Sub department:
Tropical Medicine
Role:
Author
ORCID:
0000-0001-9189-7244
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Sub department:
Tropical Medicine
Role:
Author
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Lee Ka Shing Foundation More from this funder
Publisher:
Wellcome Trust Publisher's website
Journal:
Wellcome Open Research Journal website
Volume:
3
Pages:
132
Publication date:
2018-11-21
Acceptance date:
2018-10-15
DOI:
EISSN:
2398-502X
Source identifiers:
935848
Keywords:
Pubs id:
pubs:935848
UUID:
uuid:6a937401-a45a-43fb-9e4a-5429b29e42c5
Local pid:
pubs:935848
Deposit date:
2018-11-15

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