Journal article icon

Journal article

Structural basis for reversible photoswitching in Dronpa.

Abstract:

Dronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching properties. It may be reversibly switched between a fluorescent on-state and a nonfluorescent off-state by irradiation with light. To elucidate the molecular basis of the switching mechanism, we generated reversibly switchable Dronpa protein crystals. Using these crystals we determined the elusive dark-state structure of Dronpa at 1.95-A resolution. We found that the photoswitching results in a cis-tran...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1073/pnas.0700629104

Authors


Andresen, M More by this author
Trowitzsch, S More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Expand authors...
Journal:
Proceedings of the National Academy of Sciences of the United States of America
Volume:
104
Issue:
32
Pages:
13005-13009
Publication date:
2007-08-05
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
URN:
uuid:6a4e94f3-1181-4ee7-a372-5d6000c6f20c
Source identifiers:
222175
Local pid:
pubs:222175

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP