Inducible-expression plasmid for Rhodobacter sphaeroides and Paracoccus denitrificans.

Journal article

We have developed a stable isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible-expression plasmid, pIND4, which allows graduated levels of protein expression in the alphaproteobacteria Rhodobacter sphaeroides and Paracoccus denitrificans. pIND4 confers kanamycin resistance and combines the stable replicon of pMG160 with the lacI(q) gene from pYanni3 and the lac promoter, P(A1/04/03), from pJBA24.

Authors: Ind, A; Porter, S; Brown, M; Byles, E; de Beyer, J

• Publication status: Published
• Journal: Applied and environmental microbiology
• Volume: 75
• Issue: 20
• Pages: 6613-6615
• Publication date: 2009-10-01
• DOI: 10.1128/aem.01587-09
• EISSN: 1098-5336
• ISSN: 0099-2240
• Language: English
• Keywords: Gene Expression; Promoter Regions, Genetic; Rhodobacter sphaeroides; Molecular Sequence Data; Paracoccus denitrificans; Lac Operon; Genetic Vectors; Chromosome Mapping; Replicon; Isopropyl Thiogalactoside; Kanamycin Resistance; Genes, Bacterial; Plasmids