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Investigation of the substrate range of CYP199A4: modification of the partition between hydroxylation and desaturation activities by substrate and protein engineering.

Abstract:

The cytochrome P450 enzyme CYP199A4, from Rhodopseudomonas palustris HaA2, can efficiently demethylate 4-methoxybenzoic acid. It is also capable of oxidising a range of other related substrates. By investigating substrates with different substituents and ring systems we have been able to show that the carboxylate group and the nature of the ring system and the substituent are all important for optimal substrate binding and activity. The structures of the veratric acid, 2-naphthoic acid and in...

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Publication status:
Published

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Publisher copy:
10.1002/chem.201202776

Authors


Gentleman, AS More by this author
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Journal:
Chemistry (Weinheim an der Bergstrasse, Germany)
Volume:
18
Issue:
52
Pages:
16677-16688
Publication date:
2012-12-05
DOI:
EISSN:
1521-3765
ISSN:
0947-6539
URN:
uuid:6a13ac07-b832-49b6-b3e0-41e34d9f4b75
Source identifiers:
358300
Local pid:
pubs:358300

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