TCR- and CD28-mediated recruitment of phosphodiesterase 4 to lipid rafts potentiates TCR signaling.

Journal article

Ligation of the TCR along with the coreceptor CD28 is necessary to elicit T cell activation in vivo, whereas TCR triggering alone does not allow a full T cell response. Upon T cell activation of human peripheral blood T cells, we found that the majority of cAMP was generated in T cell lipid rafts followed by activation of protein kinase A. However, upon TCR and CD28 coligation, beta-arrestin in complex with cAMP-specific phosphodiesterase 4 (PDE4) was recruited to lipid rafts which down-regul...

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Authors: Abrahamsen, H; Baillie, G; Ngai, J; Vang, T; Nika, K

• Publication status: Published
• Journal: Journal of Immunology
• Volume: 173
• Issue: 8
• Pages: 4847-4858
• Publication date: 2004-10-01
• DOI: 10.4049/jimmunol.173.8.4847
• EISSN: 1550-6606
• ISSN: 0022-1767
• Language: English
• Keywords: Humans; Membrane Microdomains; Cyclic Nucleotide Phosphodiesterases, Type 4; Signal Transduction; 1-Methyl-3-isobutylxanthine; Antigens, CD28; Arrestins; 3',5'-Cyclic-AMP Phosphodiesterases; T-Lymphocytes; Lymphocyte Activation; Cyclic AMP; Receptors, Antigen, T-Cell; Cyclic AMP-Dependent Protein Kinases