A mass spectrometry-based hybrid method for structural modeling of protein complexes.

Journal article

We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.

Authors: Politis, A; Stengel, F; Hall, Z; Hernández, H; Leitner, A

• Publication status: Published
• Journal: Nature methods
• Volume: 11
• Issue: 4
• Pages: 403-406
• Publication date: 2014-04-01
• DOI: 10.1038/nmeth.2841
• EISSN: 1548-7105
• ISSN: 1548-7091
• Language: English
• Keywords: Mass Spectrometry; Models, Molecular; Protein Conformation; Animals; Proteins