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A mass spectrometry-based hybrid method for structural modeling of protein complexes.

Abstract:
We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.
Publication status:
Published

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Publisher copy:
10.1038/nmeth.2841

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Journal:
Nature methods More from this journal
Volume:
11
Issue:
4
Pages:
403-406
Publication date:
2014-04-01
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Language:
English
Keywords:
Pubs id:
pubs:449185
UUID:
uuid:69d4ef8b-e240-4e29-a478-d19be5ed2061
Local pid:
pubs:449185
Source identifiers:
449185
Deposit date:
2014-02-24

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