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A genetically encoded, fluorescent indicator for cyclic AMP in living cells.

Abstract:

Cyclic AMP controls several signalling cascades within cells, and changes in the amounts of this second messenger have an essential role in many cellular events. Here we describe a new methodology for monitoring the fluctuations of cAMP in living cells. By tagging the cAMP effector protein kinase A with two suitable green fluorescent protein mutants, we have generated a probe in which the fluorescence resonance energy transfer between the two fluorescent moieties is dependent on the levels of...

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Publisher copy:
10.1038/71345

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Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
Journal:
Nature cell biology More from this journal
Volume:
2
Issue:
1
Pages:
25-29
Publication date:
2000-01-01
DOI:
EISSN:
1476-4679
ISSN:
1465-7392

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