A genetically encoded, fluorescent indicator for cyclic AMP in living cells.

Journal article

Cyclic AMP controls several signalling cascades within cells, and changes in the amounts of this second messenger have an essential role in many cellular events. Here we describe a new methodology for monitoring the fluctuations of cAMP in living cells. By tagging the cAMP effector protein kinase A with two suitable green fluorescent protein mutants, we have generated a probe in which the fluorescence resonance energy transfer between the two fluorescent moieties is dependent on the levels of...

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Authors: Zaccolo, M; De Giorgi, F; Cho, C; Feng, L; Knapp, T

• Journal: Nature cell biology
• Volume: 2
• Issue: 1
• Pages: 25-29
• Publication date: 2000-01-01
• DOI: 10.1038/71345
• EISSN: 1476-4679
• ISSN: 1465-7392
• Language: English
• Keywords: Green Fluorescent Proteins; Microscopy, Fluorescence; Forskolin; Transfection; Luminescent Proteins; Adrenergic beta-Agonists; beta-Lactamases; Second Messenger Systems; Indicators and Reagents; Genes, Reporter; Norepinephrine; COS Cells; Bucladesine; Mutagenesis; Cyclic AMP Response Element-Binding Protein; Isoproterenol; Cyclic AMP; CHO Cells; Kidney; Propranolol; Cricetinae; Cyclic AMP-Dependent Protein Kinases; Adrenergic alpha-Agonists; Gene Expression Regulation, Enzymologic; Adrenergic beta-Antagonists; Humans; Animals; Cytogenetic Analysis