Modulation of the osteoclast Ca2+ receptor by extracellular protons: possible linkage between Ca2+ sensing and extracellular acidification.

Journal article

We report a sensitivity of the osteoclast cell surface Ca2+ receptor to extracellular protons. Freshly isolated rat osteoclasts were exposed to the known agonists of the Ca2+ receptor, Ca2+ and Ni2+, in extracellular solutions set at different pH values. Decreasing the extracellular pH from 7.8 to 4.0 units markedly potentiated the cytosolic Ca2+ signals elicited in response to Ca2+ receptor activation by either Ni2+ (50 microM, 500 microM or 5 mM) or Ca2+ (5 mM). Each response consisted of a...

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Authors: Adebanjo, O; Shankar, V; Pazianas, M; Zaidi, A; Simon, B

• Journal: Biochemical and biophysical research communications
• Volume: 199
• Issue: 2
• Pages: 742-747
• Publication date: 1994-03-01
• DOI: 10.1006/bbrc.1994.1291
• EISSN: 1090-2104
• ISSN: 0006-291X
• Language: English
• Keywords: Rats, Wistar; Nickel; Cytosol; Egtazic Acid; Cells, Cultured; Rats; Spectrometry, Fluorescence; Animals; Calcium; Cell Membrane; Osteoclasts; Animals, Newborn; Calcium-Binding Proteins; Hydrogen-Ion Concentration; Fura-2