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rsEGFP2 enables fast RESOLFT nanoscopy of living cells

Abstract:

The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster swit...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

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Publisher copy:
10.7554/eLife.00248.001

Authors


Grotjohann, T More by this author
Brakemann, T More by this author
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Subgroup:
Weatherall Institute of Molecular Medicine
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Publisher:
eLife Sciences Publications Publisher's website
Journal:
eLife Journal website
Volume:
2012
Issue:
1
Pages:
Article: e00248
Publication date:
2012-12-31
Acceptance date:
2012-11-19
DOI:
EISSN:
2050-084X
URN:
uuid:693251a3-7ffc-46b8-a129-d41f5f008aa0
Source identifiers:
374299
Local pid:
pubs:374299

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