- Abstract:
-
The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster swit...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's Version
- Files:
-
-
(pdf, 3.6mb)
-
- Publisher copy:
- 10.7554/eLife.00248.001
-
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- Publisher:
- eLife Sciences Publications Publisher's website
- Journal:
- eLife Journal website
- Volume:
- 2012
- Issue:
- 1
- Pages:
- Article: e00248
- Publication date:
- 2012-12-31
- Acceptance date:
- 2012-11-19
- DOI:
- EISSN:
-
2050-084X
- URN:
-
uuid:693251a3-7ffc-46b8-a129-d41f5f008aa0
- Source identifiers:
-
374299
- Local pid:
- pubs:374299
- Language:
- English
- Keywords:
- Copyright holder:
- Grotjohann et al.
- Copyright date:
- 2012
- Notes:
- © 2012, Grotjohann et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Journal article
rsEGFP2 enables fast RESOLFT nanoscopy of living cells
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