Journal article
rsEGFP2 enables fast RESOLFT nanoscopy of living cells
- Abstract:
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The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster swit...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, pdf, 3.6MB)
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- Publisher copy:
- 10.7554/eLife.00248.001
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Authors
Bibliographic Details
- Publisher:
- eLife Sciences Publications Publisher's website
- Journal:
- eLife Journal website
- Volume:
- 2012
- Issue:
- 1
- Article number:
- e00248
- Publication date:
- 2012-12-31
- Acceptance date:
- 2012-11-19
- DOI:
- EISSN:
-
2050-084X
- Source identifiers:
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374299
Item Description
- Language:
- English
- Keywords:
- UUID:
-
uuid:693251a3-7ffc-46b8-a129-d41f5f008aa0
- Local pid:
- pubs:374299
- Deposit date:
- 2013-11-16
Terms of use
- Copyright holder:
- Grotjohann et al
- Copyright date:
- 2012
- Notes:
- © 2012, Grotjohann et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
- Licence:
- CC Attribution (CC BY)
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