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Radiation damage and dose limits in serial synchrotron crystallography at cryo- and room temperatures

Abstract:

Radiation damage limits the accuracy of macromolecular structures in X-ray crystallography. Cryogenic (cryo-) cooling reduces the global radiation damage rate and, therefore, became the method of choice over the past decades. The recent advent of serial crystallography, which spreads the absorbed energy over many crystals, thereby reducing damage, has rendered room temperature (RT) data collection more practical and also extendable to microcrystals, both enabling and requiring the study of sp...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher:
National Academy of Sciences
Journal:
Proceedings of the National Academy of Sciences More from this journal
Volume:
117
Issue:
8
Pages:
4142-4151
Publication date:
2020-02-25
Acceptance date:
2020-01-08
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
Language:
English
Keywords:
Pubs id:
pubs:1081316
UUID:
uuid:6928684f-decf-45a9-b5fd-7129f89b6c62
Local pid:
pubs:1081316
Source identifiers:
1081316
Deposit date:
2020-01-09

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