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Inclusion of PF68 surfactant improves stability of rAAV titer when passed through a surgical device used in retinal gene therapy

Abstract:

Recent advances in recombinant adeno-associated virus (rAAV) gene therapy for choroideremia show gene replacement to be a promising approach. It is, however, well known that contact of vector solution with plastic materials in the surgical device may result in non-specific adsorption with resulting loss of physical titer and/or level of protein expression and activity. Here we assessed the biocompatibility and stability of rAAV2-REP1 (Rab Escort Protein-1) before and following passage through...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.omtm.2019.11.005

Authors


More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Clinical Neurosciences
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Clinical Neurosciences
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Clinical Neurosciences
Role:
Author
Publisher:
Cell Press Publisher's website
Journal:
Molecular Therapy - Methods and Clinical Development Journal website
Volume:
17
Pages:
99-106
Publication date:
2019-11-20
Acceptance date:
2019-11-04
DOI:
ISSN:
2329-0501
Pmid:
31890744
Language:
English
Keywords:
Pubs id:
pubs:1080249
UUID:
uuid:68d2ec3a-7659-49e5-8ece-743e4aa36dd9
Local pid:
pubs:1080249
Source identifiers:
1080249
Deposit date:
2020-01-15

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