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Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions.

Abstract:

The freeze denaturation of model proteins, LDH, ADH, and catalase, was investigated in absence of cryoprotectants using a microcryostage under well-controlled freezing and thawing rates. Most of the experimental data were obtained from a study using a dilute solution with an enzyme concentration of 0.025 g/l. The dependence of activity recovery of proteins on the freezing and thawing rates showed a reciprocal and independent effect, that is, slow freezing (at a freezing rate about 1 degrees C...

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Publication status:
Published

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Publisher copy:
10.1002/bit.10612

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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Role:
Author
Journal:
Biotechnology and bioengineering
Volume:
82
Issue:
6
Pages:
684-690
Publication date:
2003-06-05
DOI:
EISSN:
1097-0290
ISSN:
0006-3592
URN:
uuid:685f246d-8db3-4be4-bb0e-c5788a25b92e
Source identifiers:
61957
Local pid:
pubs:61957

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