Journal article
Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions.
- Abstract:
-
The freeze denaturation of model proteins, LDH, ADH, and catalase, was investigated in absence of cryoprotectants using a microcryostage under well-controlled freezing and thawing rates. Most of the experimental data were obtained from a study using a dilute solution with an enzyme concentration of 0.025 g/l. The dependence of activity recovery of proteins on the freezing and thawing rates showed a reciprocal and independent effect, that is, slow freezing (at a freezing rate about 1 degrees C...
Expand abstract
- Publication status:
- Published
Actions
Access Document
- Publisher copy:
- 10.1002/bit.10612
Why is the content I wish to access not available via ORA?
Bibliographic data (the information relating to research outputs) and full-text items (e.g. articles, theses, reports, etc.) arrive in ORA from several different sources. Unfortunately we are not able to make available the full-text for every research output.
Please contact the ORA team (
) if you have queries regarding unavailable content OR if you are aware of a full-text copy we can make available.
) if you have queries regarding unavailable content OR if you are aware of a full-text copy we can make available.
Content may be unavailable for the following four reasons
-
Version unsuitable
-
We have not obtained a suitable full-text for a given research output. See this page for more information.
-
Recently completed
-
Sometimes content is held in ORA but is unavailable for a fixed period of time to comply with the policies and wishes of rights holders.
-
Permissions
-
All content made available in ORA should comply with relevant rights, such as copyright. See this page for more information.
-
Clearance
-
Some thesis volumes scanned as part of the digitisation scheme funded by Dr Leonard Polonsky are currently unavailable due to sensitive material or uncleared third-party copyright content. We are attempting to contact authors whose theses are affected.
Alternative access to the full-text
You may be able to access the full-text directly from the publisher's website using the 'Publisher Copy' link in the 'Links & Downloads' box from a research output's ORA record page. This method may require an institutional or individual subscription to the journal/resource.
Authors
Bibliographic Details
- Journal:
- Biotechnology and bioengineering
- Volume:
- 82
- Issue:
- 6
- Pages:
- 684-690
- Publication date:
- 2003-06-01
- DOI:
- EISSN:
-
1097-0290
- ISSN:
-
0006-3592
- Source identifiers:
-
61957
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:61957
- UUID:
-
uuid:685f246d-8db3-4be4-bb0e-c5788a25b92e
- Local pid:
- pubs:61957
- Deposit date:
- 2013-11-16
Terms of use
- Copyright date:
- 2003
If you are the owner of this record, you can report an update to it here: Report update to this record