- Abstract:
-
The freeze denaturation of model proteins, LDH, ADH, and catalase, was investigated in absence of cryoprotectants using a microcryostage under well-controlled freezing and thawing rates. Most of the experimental data were obtained from a study using a dilute solution with an enzyme concentration of 0.025 g/l. The dependence of activity recovery of proteins on the freezing and thawing rates showed a reciprocal and independent effect, that is, slow freezing (at a freezing rate about 1 degrees C...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1002/bit.10612
-
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- Journal:
- Biotechnology and bioengineering
- Volume:
- 82
- Issue:
- 6
- Pages:
- 684-690
- Publication date:
- 2003-06-05
- DOI:
- EISSN:
-
1097-0290
- ISSN:
-
0006-3592
- URN:
-
uuid:685f246d-8db3-4be4-bb0e-c5788a25b92e
- Source identifiers:
-
61957
- Local pid:
- pubs:61957
- Copyright date:
- 2003
Journal article
Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions.
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