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Translocation of sigma(70) with RNA polymerase during transcription: fluorescence resonance energy transfer assay for movement relative to DNA.

Abstract:

Using fluorescence resonance energy transfer, we show that, in the majority of transcription complexes, sigma(70) is not released from RNA polymerase upon transition from initiation to elongation, but, instead, remains associated with RNA polymerase and translocates with RNA polymerase. The results argue against the presumption that there are necessary subunit-composition differences, and corresponding necessary mechanistic differences, in initiation and elongation. The methods of this report...

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Journal:
Cell
Volume:
106
Issue:
4
Pages:
453-463
Publication date:
2001-08-05
DOI:
EISSN:
1097-4172
ISSN:
0092-8674
URN:
uuid:684cfd78-0374-4af6-a6eb-9461122f116b
Source identifiers:
31253
Local pid:
pubs:31253

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