Journal article
Translocation of sigma(70) with RNA polymerase during transcription: fluorescence resonance energy transfer assay for movement relative to DNA.
- Abstract:
-
Using fluorescence resonance energy transfer, we show that, in the majority of transcription complexes, sigma(70) is not released from RNA polymerase upon transition from initiation to elongation, but, instead, remains associated with RNA polymerase and translocates with RNA polymerase. The results argue against the presumption that there are necessary subunit-composition differences, and corresponding necessary mechanistic differences, in initiation and elongation. The methods of this report...
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- Publisher copy:
- 10.1016/s0092-8674(01)00464-0
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Bibliographic Details
- Journal:
- Cell
- Volume:
- 106
- Issue:
- 4
- Pages:
- 453-463
- Publication date:
- 2001-08-01
- DOI:
- EISSN:
-
1097-4172
- ISSN:
-
0092-8674
- Source identifiers:
-
31253
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:31253
- UUID:
-
uuid:684cfd78-0374-4af6-a6eb-9461122f116b
- Local pid:
- pubs:31253
- Deposit date:
- 2012-12-20
Terms of use
- Copyright date:
- 2001
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