- Abstract:
-
Using fluorescence resonance energy transfer, we show that, in the majority of transcription complexes, sigma(70) is not released from RNA polymerase upon transition from initiation to elongation, but, instead, remains associated with RNA polymerase and translocates with RNA polymerase. The results argue against the presumption that there are necessary subunit-composition differences, and corresponding necessary mechanistic differences, in initiation and elongation. The methods of this report...
Expand abstract - Publisher copy:
- 10.1016/s0092-8674(01)00464-0
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- Journal:
- Cell
- Volume:
- 106
- Issue:
- 4
- Pages:
- 453-463
- Publication date:
- 2001-08-05
- DOI:
- EISSN:
-
1097-4172
- ISSN:
-
0092-8674
- URN:
-
uuid:684cfd78-0374-4af6-a6eb-9461122f116b
- Source identifiers:
-
31253
- Local pid:
- pubs:31253
- Copyright date:
- 2001
Journal article
Translocation of sigma(70) with RNA polymerase during transcription: fluorescence resonance energy transfer assay for movement relative to DNA.
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