Journal article
Reversible photoswitching enables single-molecule fluorescence fluctuation spectroscopy at high molecular concentration.
- Abstract:
-
We demonstrate that photoswitchable markers enable fluorescence fluctuation spectroscopy at high molecular concentration. Reversible photoswitching allows precise control of the density of fluorescing entities, because the equilibrium between the fluorescent ON- and the dark OFF-state can be shifted through optical irradiation at a specific wavelength. Depending on the irradiation intensity, the concentration of the ON-state markers can be up to 1,000 times lower than the actual concentration...
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- Publication status:
- Published
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- Publisher copy:
- 10.1002/jemt.20505
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Bibliographic Details
- Journal:
- Microscopy research and technique
- Volume:
- 70
- Issue:
- 12
- Pages:
- 1003-1009
- Publication date:
- 2007-12-01
- DOI:
- EISSN:
-
1097-0029
- ISSN:
-
1059-910X
- Source identifiers:
-
222177
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:222177
- UUID:
-
uuid:6845d7fd-1085-45d6-8bdd-58e7f2566f94
- Local pid:
- pubs:222177
- Deposit date:
- 2012-12-19
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- Copyright date:
- 2007
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