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Reversible photoswitching enables single-molecule fluorescence fluctuation spectroscopy at high molecular concentration.

Abstract:

We demonstrate that photoswitchable markers enable fluorescence fluctuation spectroscopy at high molecular concentration. Reversible photoswitching allows precise control of the density of fluorescing entities, because the equilibrium between the fluorescent ON- and the dark OFF-state can be shifted through optical irradiation at a specific wavelength. Depending on the irradiation intensity, the concentration of the ON-state markers can be up to 1,000 times lower than the actual concentration...

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Publication status:
Published

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Publisher copy:
10.1002/jemt.20505

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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
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Journal:
Microscopy research and technique
Volume:
70
Issue:
12
Pages:
1003-1009
Publication date:
2007-12-01
DOI:
EISSN:
1097-0029
ISSN:
1059-910X
Source identifiers:
222177
Language:
English
Keywords:
Pubs id:
pubs:222177
UUID:
uuid:6845d7fd-1085-45d6-8bdd-58e7f2566f94
Local pid:
pubs:222177
Deposit date:
2012-12-19

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