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Re-purposing Ac/Ds transgenic system for CRISPR/dCas9 modulation of enhancers and non-coding RNAs in zebrafish

Abstract:

Due to its genetic amenability coupled with recent advances in genome editing, the zebrafish serves as an excellent model to examine the function of both coding and non-coding elements. Recently, the non-coding genome has gained prominence due to its critical role in development and disease. Here, we have re-purposed the Ac/Ds maize transposition system to reliably screen and efficiently characterise zebrafish enhancers, with or without germline propagation. We further utilised the system to ...

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Publication status:
Not Published
Peer review status:
Not peer reviewed
Version:
Author's Original

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM Clinical Laboratory Sciences
ORCID:
0000-0002-2547-3694
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
Senanayake, U More by this author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Medical School Offices
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
Oxford college:
Jesus College
ORCID:
0000-0002-9862-7332
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Publication date:
2018-10-23
Pubs id:
pubs:942188
URN:
uri:683a64ac-81fd-41d8-b816-17836e86ebf2
UUID:
uuid:683a64ac-81fd-41d8-b816-17836e86ebf2
Local pid:
pubs:942188

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