Unlinking chromosome catenanes in vivo by site-specific recombination.

Journal article

A challenge for chromosome segregation in all domains of life is the formation of catenated progeny chromosomes, which arise during replication as a consequence of the interwound strands of the DNA double helix. Topoisomerases play a key role in DNA unlinking both during and at the completion of replication. Here we report that chromosome unlinking can instead be accomplished by multiple rounds of site-specific recombination. We show that step-wise, site-specific recombination by XerCD-dif or...

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Authors: Grainge, I; Bregu, M; Vazquez, M; Sivanathan, V; Ip, S

• Publication status: Published
• Journal: EMBO journal
• Volume: 26
• Issue: 19
• Pages: 4228-4238
• Publication date: 2007-10-01
• DOI: 10.1038/sj.emboj.7601849
• EISSN: 1460-2075
• ISSN: 0261-4189
• Language: English
• Keywords: Escherichia coli; Escherichia coli Proteins; DNA Topoisomerase IV; DNA Replication; Integrases; DNA, Catenated; Membrane Proteins; Recombination, Genetic; Chromosomes, Bacterial