PML bodies control the nuclear dynamics and function of the CHFR mitotic checkpoint protein.

Journal article

Nuclear foci containing the promyelocytic leukemia protein (PML bodies), which occur in most cells, play a role in tumor suppression. Here, we demonstrate that CHFR, a mitotic checkpoint protein frequently inactivated in human cancers, is a dynamic component of PML bodies. Intermolecular fluorescence resonance energy transfer analysis identified a distinct fraction of CHFR that interacts with PML in living cells. This interaction modulates the nuclear distribution and mobility of CHFR. A tran...

Expand abstract

Authors: Daniels, M; Marson, A; Venkitaraman, A

• Publication status: Published
• Journal: Nature structural and molecular biology
• Volume: 11
• Issue: 11
• Pages: 1114-1121
• Publication date: 2004-11-01
• DOI: 10.1038/nsmb837
• EISSN: 1545-9985
• ISSN: 1545-9993
• Language: English
• Keywords: Cell Line; Models, Genetic; Cell Cycle; Xenopus laevis; Immunoprecipitation; Protein Binding; Green Fluorescent Proteins; Transgenes; Intranuclear Inclusion Bodies; Cell Nucleus; Transfection; Time Factors; Cloning, Molecular; Mutation; Neoplasm Proteins; Fluorescence Resonance Energy Transfer; Microscopy, Fluorescence; Xenopus; DNA, Complementary; Humans; Transcription Factors; Tumor Suppressor Proteins; Chromosome Aberrations; Recombinant Fusion Proteins; Cell Cycle Proteins; Mitosis; Nuclear Proteins; Mice; Animals