EXCHANGE OF THE VALINE-2-H IN THE BIOSYNTHESIS OF L-DELTA-(ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VALINE

Journal article

Incubation of [2-2H]-valine with purified ACV synthetase from both Cephalosporium acremonium and Streptomyces clavuligerus produced L-δ-(α-aminoadipoyl)-L-cysteinyl-D-valine (ACV), determined by the essentially complete (>95%) loss of deuterium from the α position of the incorporated valine. Incubations with deuterium oxide/water as solvent produced ACV with significant incorporation of deuterium into the valinyl residue. These observations confirm the prior proposal that a single multifun...

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Authors: Baldwin, J; Byford, M; Field, R; Shiau, C; Sobey, W

• Publication status: Published
• Journal: TETRAHEDRON
• Volume: 49
• Issue: 15
• Pages: 3221-3226
• Publication date: 1993-04-09
• DOI: 10.1016/S0040-4020(01)89904-X
• ISSN: 0040-4020
• Language: English
• Keywords: DEUTERIUM EXCHANGE; L-DELTA-(ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VALINE; PENICILLIN; BIOSYNTHESIS; EPIMERIZATION