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A structural study of isolated mammalian centrioles using negative staining electron microscopy.

Abstract:
We have used a combination of centrifugation onto electron microscope grids and negative staining to study the structure of isolated mammalian centrioles. The technique relies on visualisation of structural detail by use of a goldthioglucose negative stain. The approach provides an easy structural definition of the mature and immature centriole and has revealed some novel proximal projections on the mature centriole. The rapid technique should prove of use in future analyses of centriolar structure and biochemistry.
Publication status:
Published

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Publisher copy:
10.1006/jsbi.1996.0086

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Journal:
Journal of structural biology
Volume:
117
Issue:
3
Pages:
222-226
Publication date:
1996
DOI:
EISSN:
1095-8657
ISSN:
1047-8477
URN:
uuid:67b90332-3659-4247-9302-8b56a33cc533
Source identifiers:
28388
Local pid:
pubs:28388

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