Journal article
In silico guided reconstruction and analysis of ICAM-1-binding var genes from 1 Plasmodium falciparum
- Abstract:
- The Plasmodium falciparum variant surface antigen PfEMP1 expressed on the surface of infected erythrocytes is thought to play a major role in the pathology of severe malaria. As the sequence pool of the var genes encoding PfEMP1 expands there are opportunities, despite the high degree of sequence diversity demonstrated by this gene family, to reconstruct full-length var genes from small sequence tags generated from patient isolates. To test whether this is possible we have used a set of recently laboratory adapted ICAM-1-binding parasite isolates to generate sequence tags and, from these, to identify the full-length PfEMP1 being expressed by them. In a subset of the strains available we were able to produce validated, full-length var gene sequences and use these to conduct biophysical analyses of the ICAM-1 binding regions.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 2.7MB, Terms of use)
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- Publisher copy:
- 10.1038/s41598-018-21591-8
Authors
- Publisher:
- Nature Publishing Group
- Journal:
- Scientific Reports More from this journal
- Volume:
- 8
- Pages:
- 3282
- Publication date:
- 2018-02-01
- Acceptance date:
- 2018-02-07
- DOI:
- EISSN:
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2045-2322
- ISSN:
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2045-2322
- Keywords:
- Pubs id:
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pubs:823120
- UUID:
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uuid:67b6c1dc-71d1-4ade-b75e-414459a22da6
- Local pid:
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pubs:823120
- Source identifiers:
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823120
- Deposit date:
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2018-02-08
Terms of use
- Copyright holder:
- Newbold et al
- Copyright date:
- 2018
- Notes:
- © The Author(s) 2018 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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