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Journal article

Development of homogeneous luminescence assays for histone demethylase catalysis and binding.

Abstract:

Covalent modifications to histones play important roles in chromatin dynamics and the regulation of gene expression. The JumonjiC (JmjC)-containing histone demethylases (HDMs) catalyze the demethylation of methylated lysine residues on histone tails. Here we report the development of homogeneous luminescence-based assay methods for measuring the catalytic activity and the binding affinities of peptides to HDMs. The assays use amplified luminescent proximity homogeneous assay (ALPHA) technolog...

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Journal:
Analytical Biochemistry
Volume:
404
Issue:
1
Pages:
86-93
Publication date:
2010-01-01
ISSN:
1096-0309
Language:
English
Subjects:
UUID:
uuid:678a5d84-a0c5-4de0-afb3-2505bfd4d079
Local pid:
SGC:20435012
Deposit date:
2011-08-18

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