Journal article
Development of homogeneous luminescence assays for histone demethylase catalysis and binding.
- Abstract:
-
Covalent modifications to histones play important roles in chromatin dynamics and the regulation of gene expression. The JumonjiC (JmjC)-containing histone demethylases (HDMs) catalyze the demethylation of methylated lysine residues on histone tails. Here we report the development of homogeneous luminescence-based assay methods for measuring the catalytic activity and the binding affinities of peptides to HDMs. The assays use amplified luminescent proximity homogeneous assay (ALPHA) technolog...
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Bibliographic Details
- Journal:
- Analytical Biochemistry
- Volume:
- 404
- Issue:
- 1
- Pages:
- 86-93
- Publication date:
- 2010-01-01
- ISSN:
-
1096-0309
Item Description
- Language:
- English
- Subjects:
- UUID:
-
uuid:678a5d84-a0c5-4de0-afb3-2505bfd4d079
- Local pid:
- SGC:20435012
- Deposit date:
- 2011-08-18
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- Copyright holder:
- Wellcome Trust. Canadian Institutes of Health Research
- Copyright date:
- 2010
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