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Journal article

Label-free discrimination of rhizobial bacteroids and mutants by single-cell Raman microspectroscopy

Abstract:

Symbiotic rhizobia in legume account for a large portion of nitrogen fixation in the biosphere. Nitrogen fixation is an energy-demanding process requiring tight control of metabolism and redox state. It is of great interest to understand the bacteroid differentiation process and the roles of energy storage molecules, such as glycogen and polyhydroxybutyrate (PHB), in maintaining the Rhizobium-legume symbioses. Traditional biochemical assays for checking phenotypic changes of mutants require a...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1021/acs.analchem.7b01160

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Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Role:
Author
More from this funder
Name:
Natural Environment Research Council
Grant:
NE/M002934/1
More from this funder
Name:
Engineering and Physical Sciences Research Council
Grant:
EP/M002403/1,EP/M02833X/1
EP/M02833X/1
Publisher:
American Chemical Society
Journal:
Analytical Chemistry More from this journal
Volume:
89
Issue:
12
Pages:
6336–6340
Publication date:
2017-05-25
Acceptance date:
2017-05-25
DOI:
EISSN:
1086-4377
ISSN:
0003-2700
Keywords:
Pubs id:
pubs:697569
UUID:
uuid:6753b599-c7bd-4270-946d-d71480535f95
Local pid:
pubs:697569
Source identifiers:
697569
Deposit date:
2017-05-28

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