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Journal article

Mass spectrometry of intact membrane protein complexes.

Abstract:

Mass spectrometry (MS) of intact soluble protein complexes has emerged as a powerful technique to study the stoichiometry, structure-function and dynamics of protein assemblies. Recent developments have extended this technique to the study of membrane protein complexes, where it has already revealed subunit stoichiometries and specific phospholipid interactions. Here we describe a protocol for MS of membrane protein complexes. The protocol begins with the preparation of the membrane protein c...

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Publication status:
Published

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Publisher copy:
10.1038/nprot.2013.024

Authors


Journal:
Nature protocols
Volume:
8
Issue:
4
Pages:
639-651
Publication date:
2013-04-01
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Source identifiers:
387759
Language:
English
Keywords:
Pubs id:
pubs:387759
UUID:
uuid:6727c03d-398c-4642-835c-f853200ab0fe
Local pid:
pubs:387759
Deposit date:
2013-11-17

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