Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy.

Journal article

Reversibly switchable fluorescent proteins (RSFPs) are GFP-like proteins that may be repeatedly switched by irradiation with light from a fluorescent to a nonfluorescent state, and vice versa. They can be utilized as genetically encodable probes and bear large potential for a wide array of applications, in particular for new protein tracking schemes and subdiffraction resolution microscopy. However, the currently described monomeric RSFPs emit only blue-green or green fluorescence; the spectr...

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Authors: Stiel, A; Andresen, M; Bock, H; Hilbert, M; Schilde, J

• Publication status: Published
• Journal: Biophysical journal
• Volume: 95
• Issue: 6
• Pages: 2989-2997
• Publication date: 2008-09-01
• DOI: 10.1529/biophysj.108.130146
• EISSN: 1542-0086
• ISSN: 0006-3495
• Language: English
• Keywords: Time Factors; Nanotechnology; Microscopy; Light; Cattle; Animals; Spectrometry, Fluorescence; Protein Engineering; Biological Markers; Luminescent Proteins; Escherichia coli; Color