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Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy.

Abstract:

Reversibly switchable fluorescent proteins (RSFPs) are GFP-like proteins that may be repeatedly switched by irradiation with light from a fluorescent to a nonfluorescent state, and vice versa. They can be utilized as genetically encodable probes and bear large potential for a wide array of applications, in particular for new protein tracking schemes and subdiffraction resolution microscopy. However, the currently described monomeric RSFPs emit only blue-green or green fluorescence; the spectr...

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Publication status:
Published

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Publisher copy:
10.1529/biophysj.108.130146

Authors


Journal:
Biophysical journal More from this journal
Volume:
95
Issue:
6
Pages:
2989-2997
Publication date:
2008-09-01
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
Language:
English
Keywords:
Pubs id:
pubs:222186
UUID:
uuid:671d4fd2-8d31-40c3-a628-daed43683a28
Local pid:
pubs:222186
Source identifiers:
222186
Deposit date:
2012-12-19

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