High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi.

Journal article

Isolates of Salmonella enterica serovar Typhi (Typhi), a human-restricted bacterial pathogen that causes typhoid, show limited genetic variation. We generated whole-genome sequences for 19 Typhi isolates using 454 (Roche) and Solexa (Illumina) technologies. Isolates, including the previously sequenced CT18 and Ty2 isolates, were selected to represent major nodes in the phylogenetic tree. Comparative analysis showed little evidence of purifying selection, antigenic variation or recombination b...

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Authors: Holt, K; Parkhill, J; Mazzoni, C; Roumagnac, P; Weill, F

• Publication status: Published
• Journal: Nature genetics
• Volume: 40
• Issue: 8
• Pages: 987-993
• Publication date: 2008-08-01
• DOI: 10.1038/ng.195
• EISSN: 1546-1718
• ISSN: 1061-4036
• Language: English
• Keywords: Humans; Phylogeny; Genomic Islands; Recombination, Genetic; Sequence Analysis, DNA; Typhoid Fever; Molecular Sequence Data; Bacteriophages; Plasmids; Evolution, Molecular; Polymorphism, Single Nucleotide; Salmonella typhi; Genome, Bacterial