The Role of zinc in the disulphide stress-regulated anti-sigma factor RsrA from Streptomyces coelicolor.

Journal article

The regulation of disulphide stress in actinomycetes such as Streptomyces coelicolor is known to involve the zinc-containing anti-sigma factor RsrA that binds and inactivates the redox-regulated sigma factor sigmaR. However, it is not known how RsrA senses disulphide stress nor what role the metal ion plays. Using in vitro assays, we show that while zinc is not required for sigmaR binding it is required for functional anti-sigma factor activity, and that it plays a critical role in modulating...

Expand abstract

Authors: Li, W; Bottrill, A; Bibb, M; Buttner, M; Paget, MS

• Journal: Journal of molecular biology
• Volume: 333
• Issue: 2
• Pages: 461-472
• Publication date: 2003-10-01
• DOI: 10.1016/j.jmb.2003.08.038
• EISSN: 1089-8638
• ISSN: 0022-2836
• Language: English
• Keywords: Circular Dichroism; Transcription Factors; Transcription, Genetic; Sigma Factor; Metalloproteins; Disulfides; Protein Conformation; Streptomyces; Zinc; Gene Expression Regulation, Bacterial; Protein Binding; Oxidative Stress; Bacterial Proteins; Models, Molecular; Alkylation; Resorcinols; Oxidation-Reduction; Sulfhydryl Compounds; Mutagenesis