Permanent human lymphoid cell lines were established successfully from peripheral blood lymphocytes which had been separated for HL-A typing and stored in liquid nitrogen for two years. Frozen lymphocytes were chosen from two siblings who were homozygous at the LA and FOUR HL-A loci. Thawed lymphocytes were transformed with EB virus produced by the marmoset lymphoid line B95-8. No chromosome abnormalities were seen on karyotypes prepared on cells from the established human lymphoid lines usin...Expand abstract
- Publication status:
- Publisher copy:
- Copyright date:
Permanent lymphoid lines from genetically marked lymphocytes: success with lymphocytes recovered from frozen storage.
If you are the owner of this record, you can report an update to it here: Report update to this record