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TRANSFORMATION OF A NITRATE REDUCTASE DEFICIENT MUTANT OF PENICILLIUM-CHRYSOGENUM WITH THE CORRESPONDING ASPERGILLUS-NIGER AND ASPERGILLUS-NIDULANS NIAD GENES

Abstract:

A heterologous gene mediated transformation system based on niaD, the structural gene encoding nitrate reductase, has been developed for Penicillium chrysogenum. Transformation frequencies of up to 20 transformants per microgram DNA were obtained using the Aspergillus nidulans gene and 9 transformants per microgram using the A. niger gene. Vector constructs carrying the A. nidulans ans-1 sequence and the A. niger niaD gene did not show increased transformation frequencies. Southern blot hybri...

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Publication status:
Published

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Publisher copy:
10.1007/BF00334383

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Publisher:
Springer-Verlag
Journal:
MOLECULAR and GENERAL GENETICS More from this journal
Volume:
216
Issue:
2-3
Pages:
408-411
Publication date:
1989-04-01
DOI:
EISSN:
1617-4623
ISSN:
0026-8925
Language:
English
Keywords:
Pubs id:
pubs:43197
UUID:
uuid:6406b6c5-5655-4f04-b277-332eca3bdecc
Local pid:
pubs:43197
Source identifiers:
43197
Deposit date:
2012-12-19

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