We developed a low-cost and reliable method of DNA extraction from as little as 1 ml of early positive mycobacterial growth indicator tube (MGIT) cultures that is suitable for whole-genome sequencing to identify mycobacterial species and predict antibiotic resistance in clinical samples. The DNA extraction method is based on ethanol precipitation supplemented by pretreatment steps with a MolYsis kit or saline wash for the removal of human DNA and a final DNA cleanup step with solid-phase reve...Expand abstract
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- American Society for Microbiology Publisher's website
- Journal of clinical microbiology Journal website
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- Votintseva et al.
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- Copyright © 2015, Votintseva et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.
Mycobacterial DNA extraction for whole-genome sequencing from early positive liquid (MGIT) cultures
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