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Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions

Abstract:

The αβ T-cell coreceptor CD4 enhances immune responses more than 1 million-fold in some assays, and yet the affinity of CD4 for its ligand, peptide-major histocompatibility class II (pMHC II) on antigen-presenting cells, is so weak that it was previously unquantifiable. Here, we report that a soluble form of CD4 failed to bind detectably to pMHC II in surface plasmon resonance-based assays, establishing a new upper limit for the solution affinity at 2.5 mM. However, when presented multival...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1073/pnas.1513918113

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Institution:
University of Oxford
Department:
Oxford, MSD, Obstetrics and Gynaecology
Role:
Author
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Institution:
University of Oxford
Department:
Oxford, MSD
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD
Role:
Author
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Funding agency for:
Dushek, O
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Funding agency for:
Dushek, O
Wellcome Trust More from this funder
Medical Research Council More from this funder
Publisher:
National Academy of Sciences Publisher's website
Journal:
Proceedings of the National Academy of Sciences Journal website
Volume:
113
Issue:
20
Pages:
5682–5687
Publication date:
2016-01-01
DOI:
EISSN:
1091-6490
URN:
uuid:63b63256-b8dc-4001-a7c1-bad76e50d316
Source identifiers:
615384
Local pid:
pubs:615384
Paper number:
20

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