Effects of the protonation state of the EEEE motif of a bacterial Na(+)-channel on conduction and pore structure.

Journal article

A distinctive feature of prokaryotic Na(+)-channels is the presence of four glutamate residues in their selectivity filter. In this study, how the structure of the selectivity filter, and the free-energy profile of permeating Na(+) ions are altered by the protonation state of Glu177 are analyzed. It was found that protonation of a single glutamate residue was enough to modify the conformation of the selectivity filter and its conduction properties. Molecular dynamics simulations revealed that...

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Authors: Furini, S; Barbini, P; Domene, C

• Publication status: Published
• Publisher: Biophysical Society
• Journal: Biophysical journal
• Volume: 106
• Issue: 10
• Pages: 2175-2183
• Publication date: 2014-05-01
• DOI: 10.1016/j.bpj.2014.04.005
• EISSN: 1542-0086
• ISSN: 0006-3495
• Language: English
• Keywords: Probability; Sodium; Protons; Sodium Channels; Molecular Dynamics Simulation; Electrophysiological Processes; Amino Acid Motifs; Bacterial Proteins; Porosity