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Measurement of glutathione levels in intact roots of Arabidopsis.

Abstract:

Levels of glutathione were measured for different cell types in roots of intact Arabidopsis seedlings after labelling with monochlorobimane to give fluorescent glutathione S-bimane (GSB) and imaging using confocal laser scanning microscopy with excitation at 442 nm. Labelling increased to a plateau in most cell types after about 15-20 min and the GSB accumulated rapidly in the vacuole. Formation of GSB in the cytoplasm was not affected by treatment with sodium azide; however, vacuolar transpo...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
Journal:
Journal of microscopy More from this journal
Volume:
198
Issue:
Pt 3
Pages:
162-173
Publication date:
2000-06-01
DOI:
EISSN:
1365-2818
ISSN:
0022-2720
Language:
English
Keywords:
Pubs id:
pubs:31693
UUID:
uuid:63a0aa97-259a-45c0-83f1-d1546f0ba336
Local pid:
pubs:31693
Source identifiers:
31693
Deposit date:
2012-12-19

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