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Journal article

Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes.

Abstract:

A sterically undemanding azide analogue of dTTP (AHP dUTP) with an alkyl chain and ethynyl attachment to the nucleobase was designed and incorporated into DNA by primer extension, reverse transcription and polymerase chain reaction (PCR). An azide-modified 523 bp PCR amplicon with all 335 thymidines replaced by AHP dU was shown to be a perfect copy of the template from which it was amplified. Replacement of thymidine with AHP dU increases duplex stability, accounting in part for the high inco...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/nar/gkw028

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Chemical Biology
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
Publisher:
Oxford University Press
Journal:
Nucleic acids research More from this journal
Pages:
gkw028-gkw028
Publication date:
2016-01-01
Acceptance date:
2016-01-11
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Language:
English
Pubs id:
pubs:600952
UUID:
uuid:63872109-8849-47e1-9cc8-b001de1250d7
Local pid:
pubs:600952
Source identifiers:
600952
Deposit date:
2016-03-01

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