Journal article
Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes.
- Abstract:
-
A sterically undemanding azide analogue of dTTP (AHP dUTP) with an alkyl chain and ethynyl attachment to the nucleobase was designed and incorporated into DNA by primer extension, reverse transcription and polymerase chain reaction (PCR). An azide-modified 523 bp PCR amplicon with all 335 thymidines replaced by AHP dU was shown to be a perfect copy of the template from which it was amplified. Replacement of thymidine with AHP dU increases duplex stability, accounting in part for the high inco...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Authors
Funding
China Scholarship Council
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Bibliographic Details
- Publisher:
- Oxford University Press Publisher's website
- Journal:
- Nucleic acids research Journal website
- Pages:
- gkw028-gkw028
- Publication date:
- 2016-01-01
- Acceptance date:
- 2016-01-11
- DOI:
- EISSN:
-
1362-4962
- ISSN:
-
0305-1048
Item Description
- Language:
- English
- Pubs id:
-
pubs:600952
- UUID:
-
uuid:63872109-8849-47e1-9cc8-b001de1250d7
- Local pid:
- pubs:600952
- Source identifiers:
-
600952
- Deposit date:
- 2016-03-01
Terms of use
- Copyright holder:
- Ren, Al-Sagheer and Brown
- Copyright date:
- 2016
- Notes:
- © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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