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Functional human α7 nicotinic acetylcholine receptor (nAChR) generated from escherichia coli

Abstract:

Human Cys-loop receptors are important therapeutic targets. High-resolution structures are essential for rational drug design, but only a few are available due to difficulties in obtaining sufficient quantities of protein suitable for structural studies. Although expression of proteins in E. coli offers advantages of high yield, low cost, and fast turnover, this approach has not been thoroughly explored for full-length human Cys-loop receptors because of the conventional wisdom that E. coli l...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1074/jbc.M116.729970

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Name:
National Institutes of Health
Grant:
R01GM066358
Publisher:
American Society for Biochemistry and Molecular Biology
Journal:
Journal of Biological Chemistry More from this journal
Volume:
291
Issue:
35
Pages:
18276-18282
Publication date:
2017-07-06
Acceptance date:
2016-07-06
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
Language:
English
Keywords:
Pubs id:
pubs:665114
UUID:
uuid:634dde21-8541-4c93-aff2-791933168784
Local pid:
pubs:665114
Source identifiers:
665114
Deposit date:
2016-12-13

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