Sensitive affimer and antibody based impedimetric label-free assays for C-reactive protein.

Journal article

C-reactive protein (CRP) is an acute phase protein whose levels are increased in many disorders. Levels greater than 3 μg/mL serum have hitherto been considered to indicate pathology, but there is increasing interest in assessments between 0.1 and 10 μg/mL, which have been found to correlate with severity of risk for cardiovascular disease. We report herein the generation of both antibody and Affimer based impedance immunoassays for CRP that are substantially more sensitive than clinically ut...

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Authors: Johnson, A; Song, Q; Ko Ferrigno, P; Bueno, P; Davis, J

• Publication status: Published
• Journal: Analytical chemistry
• Volume: 84
• Issue: 15
• Pages: 6553-6560
• Publication date: 2012-08-01
• DOI: 10.1021/ac300835b
• EISSN: 1520-6882
• ISSN: 0003-2700
• Language: English
• Keywords: Surface Plasmon Resonance; Electrodes; Antibodies, Immobilized; Polyethylene Glycols; Protein Array Analysis; Gold; C-Reactive Protein; Dielectric Spectroscopy; Immunoassay