Intracellular pH changes in isolated bovine articular chondrocytes during the loading and removal of cryoprotective agents.

Journal article

The addition and removal of a cryoprotective agent (CPA) are necessary steps in the cryopreservation of natural or engineered tissue products. However, the introduction and removal of CPAs induces dramatic chemical changes inside tissues and cells and these could cause irreversible damage. This study examined the effect of CPA loading and removal on the intracellular pH of isolated bovine articular chondrocytes using a fluorimetric technique. Chondrocytes that had been isolated from bovine ar...

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Authors: Xu, X; Cui, Z; Wilkins, R; Urban, J

• Publication status: Published
• Journal: Cryobiology
• Volume: 46
• Issue: 2
• Pages: 161-173
• Publication date: 2003-04-01
• DOI: 10.1016/s0011-2240(03)00022-1
• EISSN: 1090-2392
• ISSN: 0011-2240
• Language: English
• Keywords: Buffers; Hydrogen-Ion Concentration; Saline Solution, Hypertonic; Chondrocytes; Cryopreservation; Animals; Cell Membrane Permeability; Dimethyl Sulfoxide; Cryoprotective Agents; Metacarpophalangeal Joint; Cell Survival; Models, Biological; Glycerol; Kinetics; Cells, Cultured; Cattle