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Journal article

D-amino acid oxidase knockdown in the mouse cerebellum reduces NR2A mRNA.

Abstract:

Virus mediated RNA-interference (RNAi) is a powerful approach to study genes in vivo. Here we report a method using lentivirus-delivered RNAi to knockdown the glial enzyme, D-amino acid oxidase (DAO), in the mouse cerebellum. After initial characterisation in vitro, we achieved a 40-50% reduction of DAO mRNA in the cerebellum 7 and 28 days after a single injection of lentivirus encoding a DAO-specific, short-hairpin RNA. Injections also decreased DAO immunoreactivity (-33%). The major substra...

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Publication status:
Published

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Publisher copy:
10.1016/j.mcn.2010.08.018

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Psychiatry
Anderson, PN More by this author
Nikiforova, N More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Psychiatry
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Journal:
Molecular and cellular neurosciences
Volume:
46
Issue:
1
Pages:
167-175
Publication date:
2011-01-05
DOI:
EISSN:
1095-9327
ISSN:
1044-7431
URN:
uuid:6325c6a5-284d-4990-897c-d94b1b793056
Source identifiers:
97965
Local pid:
pubs:97965

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