- Abstract:
-
Virus mediated RNA-interference (RNAi) is a powerful approach to study genes in vivo. Here we report a method using lentivirus-delivered RNAi to knockdown the glial enzyme, D-amino acid oxidase (DAO), in the mouse cerebellum. After initial characterisation in vitro, we achieved a 40-50% reduction of DAO mRNA in the cerebellum 7 and 28 days after a single injection of lentivirus encoding a DAO-specific, short-hairpin RNA. Injections also decreased DAO immunoreactivity (-33%). The major substra...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/j.mcn.2010.08.018
-
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- Journal:
- Molecular and cellular neurosciences
- Volume:
- 46
- Issue:
- 1
- Pages:
- 167-175
- Publication date:
- 2011-01-05
- DOI:
- EISSN:
-
1095-9327
- ISSN:
-
1044-7431
- URN:
-
uuid:6325c6a5-284d-4990-897c-d94b1b793056
- Source identifiers:
-
97965
- Local pid:
- pubs:97965
- Copyright date:
- 2011
Journal article
D-amino acid oxidase knockdown in the mouse cerebellum reduces NR2A mRNA.
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