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Exosome-mediated delivery of siRNA in vitro and in vivo.

Abstract:

The use of small interfering RNAs (siRNAs) to induce gene silencing has opened a new avenue in drug discovery. However, their therapeutic potential is hampered by inadequate tissue-specific delivery. Exosomes are promising tools for drug delivery across different biological barriers. Here we show how exosomes derived from cultured cells can be harnessed for delivery of siRNA in vitro and in vivo. This protocol first describes the generation of targeted exosomes through transfection of an expr...

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Publication status:
Published

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Publisher copy:
10.1038/nprot.2012.131

Authors


El-Andaloussi, S More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics, BHF Centre of Research Excellence
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
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Journal:
Nature protocols
Volume:
7
Issue:
12
Pages:
2112-2126
Publication date:
2012-12-05
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
URN:
uuid:63119478-e7d5-4807-b899-6fd4586bd81a
Source identifiers:
363843
Local pid:
pubs:363843

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