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Thesis

Prediction, design and characterisation of metal binding sites in antibodies

Abstract:

The design and creation of a functional metalloanti body is presented. A survey of metalloprotein structures was carried out to determine the common structural features of metal binding sites. Metal ligands were then introduced into hypervariable loop 11 of the anti-lysozyme antibody HyHEL-5 and the site comprehensively modelled. The final model was used as a basis for site-directed mutagenesis and mutant antibodies were produced using a Xenopus laevis oocyte expression system. Antigen bin...

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Peer review status:
Peer reviewed

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Department:
University of Oxford
Role:
Author
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
University of Oxford
UUID:
uuid:630ede76-2db6-4e59-bdbe-d337d4fe07a9
Local pid:
polonsky:13:40
Source identifiers:
603851221
Deposit date:
2017-10-05

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