Journal article icon

Journal article

Imaging the lipid-phase-dependent pore formation of equinatoxin II in droplet interface bilayers.

Abstract:

Using phase-separated droplet interface bilayers, we observe membrane binding and pore formation of a eukaryotic cytolysin, Equinatoxin II (EqtII). EqtII activity is known to depend on the presence of sphingomyelin in the target membrane and is enhanced by lipid phase separation. By imaging the ionic flux through individual pores in vitro, we observe that EqtII pores form predominantly within the liquid-disordered phase. We observe preferential binding of labeled EqtII at liquid-ordered/liqui...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed

Actions


Access Document


Authors


Publisher:
Cell Press
Journal:
Biophysical journal More from this journal
Volume:
106
Issue:
8
Pages:
1630-1637
Publication date:
2014-04-01
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
Language:
English
Keywords:
UUID:
uuid:6307c91e-a764-45a3-8e53-2ce5fcf1ed3c
Local pid:
pubs:462167
Source identifiers:
462167
Deposit date:
2014-06-17

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP