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Effects of a hairpin polyamide on DNA melting: comparison with distamycin and Hoechst 33258.

Abstract:

We have used DNase I footprinting and fluorescence melting studies to study the interaction of the hairpin polyamide Im-Py-Py-Py-(R)H2Ngamma-Im-Py-Py-Py-beta-Dp with its preferred binding sites (5'-WGWWCW; W=A or T) and other sequences. DNase I footprinting confirmed that the ligand binds to the sequence AGAACA at nanomolar concentrations and that changing the terminal A to G causes a dramatic decrease in affinity, while there was no interaction with the reverse sequence WCWWGW. Fluorescence ...

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Publication status:
Published

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Publisher copy:
10.1016/j.bpc.2004.06.001

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Journal:
Biophysical chemistry More from this journal
Volume:
111
Issue:
3
Pages:
205-212
Publication date:
2004-11-01
DOI:
EISSN:
1873-4200
ISSN:
0301-4622
Language:
English
Keywords:
Pubs id:
pubs:400047
UUID:
uuid:62a9c33d-acd4-476e-b4bc-8c0da07806fb
Local pid:
pubs:400047
Source identifiers:
400047
Deposit date:
2013-11-16

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