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A comparative study of uracil-DNA glycosylases from human and herpes simplex virus type 1.

Abstract:

Uracil-DNA glycosylase (UNG) is the key enzyme responsible for initiation of base excision repair. We have used both kinetic and binding assays for comparative analysis of UNG enzymes from humans and herpes simplex virus type 1 (HSV-1). Steady-state fluorescence assays showed that hUNG has a much higher specificity constant (k(cat)/K(m)) compared with the viral enzyme due to a lower K(m). The binding of UNG to DNA was also studied using a catalytically inactive mutant of UNG and non-cleavable...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.m509137200

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Genomics Consortium
Role:
Author
Journal:
Journal of biological chemistry More from this journal
Volume:
281
Issue:
8
Pages:
4983-4992
Publication date:
2006-02-01
DOI:
EISSN:
1083-351X
ISSN:
0021-9258

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