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Mechanisms of luteinizing-hormone exocytosis in Staphylococcus aureus-alpha-toxin-permeabilized sheep gonadotropes.

Abstract:

We have used primary gonadotropes permeabilized with the pore-forming protein Staphylococcus aureus alpha-toxin to investigate luteinizing hormone (lutropin, LH) exocytosis. The diameter of the alpha-toxin pores (2-3 nm) allows the exchange of small molecules, whereas larger cytosolic proteins are retained. Because of the slow exchange of small molecules through the pores, we have developed a protocol which combines prolonged pre-equilibration of the permeabilized cells at 0 degrees C before ...

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Biochemical journal More from this journal
Volume:
264
Issue:
3
Pages:
901-908
Publication date:
1989-12-01
EISSN:
1470-8728
ISSN:
0264-6021
Language:
English
Keywords:
Pubs id:
pubs:14899
UUID:
uuid:624af022-1429-46e8-a463-12a79f3dbbef
Local pid:
pubs:14899
Source identifiers:
14899
Deposit date:
2012-12-19

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