Journal article icon

Journal article

Monitoring metalloproteinase activity using synthetic fluorogenic substrates.

Abstract:

Fluorogenic synthetic substrates are commonly used to monitor the activity of peptidases in vitro. This unit presents a representative protocol that employs (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L-2,3-diaminopropionyl)-Ala-Arg-NH2 (Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2) as a substrate to assay matrix metallopeptidases (MMPs). This substrate was first described for the assay of MMP-1, -2 and -3 and it is now widely used as a general MMP substrate. Protocols are gi...

Expand abstract

Actions


Access Document


Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
Journal:
Current protocols in protein science / editorial board, John E. Coligan ... [et al.]
Volume:
Chapter 21
Pages:
Unit-21.16
Publication date:
2004-11-05
DOI:
EISSN:
1934-3663
ISSN:
1934-3655
URN:
uuid:62009aaf-81f3-47ef-9423-cf8801358ca6
Source identifiers:
226573
Local pid:
pubs:226573

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP