- Abstract:
-
Fluorogenic synthetic substrates are commonly used to monitor the activity of peptidases in vitro. This unit presents a representative protocol that employs (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L-2,3-diaminopropionyl)-Ala-Arg-NH2 (Mca-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH2) as a substrate to assay matrix metallopeptidases (MMPs). This substrate was first described for the assay of MMP-1, -2 and -3 and it is now widely used as a general MMP substrate. Protocols are gi...
Expand abstract - Publisher copy:
- 10.1002/0471140864.ps2116s33
-
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- Journal:
- Current protocols in protein science / editorial board, John E. Coligan ... [et al.]
- Volume:
- Chapter 21
- Pages:
- Unit-21.16
- Publication date:
- 2004-11-05
- DOI:
- EISSN:
-
1934-3663
- ISSN:
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1934-3655
- URN:
-
uuid:62009aaf-81f3-47ef-9423-cf8801358ca6
- Source identifiers:
-
226573
- Local pid:
- pubs:226573
- Language:
- English
- Keywords:
- Copyright date:
- 2004
Journal article
Monitoring metalloproteinase activity using synthetic fluorogenic substrates.
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