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RNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicing

Abstract:

The highly intronic nature of protein coding genes in mammals necessitates a co-transcriptional splicing mechanism as revealed by mNET-seq analysis. Immunoprecipitation of MNase-digested chromatin with antibodies against RNA polymerase II (Pol II) shows that active spliceosomes (both snRNA and proteins) are complexed to Pol II S5P CTD during elongation and co-transcriptional splicing. Notably, elongating Pol II-spliceosome complexes form strong interactions with nascent transcripts, resulting...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

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Publisher copy:
10.1016/j.molcel.2018.09.004

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
Oxford college:
Lincoln College
Role:
Author
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Funding agency for:
Proudfoot, NJ
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Funding agency for:
Proudfoot, NJ
Publisher:
Elsevier Publisher's website
Journal:
Molecular Cell Journal website
Volume:
72
Issue:
2
Pages:
369-379.e4
Publication date:
2018-10-18
Acceptance date:
2018-08-31
DOI:
EISSN:
1097-4164
ISSN:
1097-2765
Pubs id:
pubs:929222
URN:
uri:61f528e3-22ea-477f-8e68-83554564febb
UUID:
uuid:61f528e3-22ea-477f-8e68-83554564febb
Local pid:
pubs:929222

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