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Isolation of potent human Fab fragments against a novel highly immunogenic region on human muscle acetylcholine receptor which protect the receptor from myasthenic autoantibodies.

Abstract:

In the autoimmune disease myasthenia gravis (MG), antibodies against the muscle nicotinic acetylcholine receptor (AChR) cause loss of functional AChR in the neuromuscular junction. To isolate AChR-specific human antibody fragments (Fab), a phage-display library was constructed from an MG patient's thymic B lymphocytes. The first Fab isolated had a low affinity for human AChR, but two sequential antibody chain shufflings using the MG donor heavy and light chain gene repertoires resulted in iso...

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Publication status:
Published

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Publisher copy:
10.1002/eji.200425671

Authors


Fostieri, E More by this author
Tzartos, SJ More by this author
Berrih-Aknin, S More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Neuroscience, Molecular Medicine
Mamalaki, A More by this author
Journal:
European journal of immunology
Volume:
35
Issue:
2
Pages:
632-643
Publication date:
2005-02-05
DOI:
EISSN:
1521-4141
ISSN:
0014-2980
URN:
uuid:615ab3fe-7305-481d-a69e-09f7225c156a
Source identifiers:
324388
Local pid:
pubs:324388

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