Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells.

Journal article

In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca2+ concentration [( Ca2+]i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K+ or the sulphonylurea tolbutamide also raised [Ca2+]i. Addition of extracellular ATP produced a transient rapid rise in [Ca2+]i. Oscillations in [Ca2+]i were observed in the presence of 10 mM glucose. Insulinoma cells responded to glucose and tolbutam...

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Authors: Kindmark, H; Köhler, M; Nilsson, T; Arkhammar, P; Wiechel, K

• Publication status: Published
• Journal: FEBS letters
• Volume: 291
• Issue: 2
• Pages: 310-314
• Publication date: 1991-10-01
• DOI: 10.1016/0014-5793(91)81309-v
• EISSN: 1873-3468
• ISSN: 0014-5793
• Language: English
• Keywords: Humans; Islets of Langerhans; Adult; Fura-2; Aged; Cytoplasm; Female; Male; Tumor Cells, Cultured; Insulinoma; Calcium