Termination of cAMP signals by Ca2+ and G(alpha)i via extracellular Ca2+ sensors: a link to intracellular Ca2+ oscillations.

Journal article

Termination of cyclic adenosine monophosphate (cAMP) signaling via the extracellular Ca(2+)-sensing receptor (CaR) was visualized in single CaR-expressing human embryonic kidney (HEK) 293 cells using ratiometric fluorescence resonance energy transfer-dependent cAMP sensors based on protein kinase A and Epac. Stimulation of CaR rapidly reversed or prevented agonist-stimulated elevation of cAMP through a dual mechanism involving pertussis toxin-sensitive Galpha(i) and the CaR-stimulated increas...

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Authors: Gerbino, A; Ruder, W; Curci, S; Pozzan, T; Zaccolo, M

• Journal: Journal of cell biology
• Volume: 171
• Issue: 2
• Pages: 303-312
• Publication date: 2005-10-01
• DOI: 10.1083/jcb.200507054
• EISSN: 1540-8140
• ISSN: 0021-9525
• Language: English
• Keywords: Humans; Pertussis Toxin; Acetylcysteine; Calcium; Erythromycin; Cyclic AMP-Dependent Protein Kinases; Cell Membrane; Biosensing Techniques; Sensitivity and Specificity; Cell Line; Receptors, Calcium-Sensing; Calcium Signaling; GTP-Binding Protein alpha Subunits, Gi-Go; Signal Transduction; Fluorescence Resonance Energy Transfer; Cyclic AMP