DnaK/DnaJ-assisted recombinant protein production in Trichoplusia ni larvae.

Journal article

The DnaK/DnaJ Escherichia coli chaperone pair, co-produced along with recombinant proteins, has been widely used to assist protein folding in bacterial cells, although with poor consensus about the ultimate effect on protein quality and its general applicability. Here, we have evaluated for the first time these bacterial proteins as folding modulators in a highly promising recombinant protein platform based on insect larvae. Intriguingly, the bacterial chaperones enhanced the solubility of a ...

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Authors: Martínez-Alonso, M; Gómez-Sebastián, S; Escribano, J; Saiz, J; Ferrer-Miralles, N

• Journal: Applied microbiology and biotechnology
• Volume: 86
• Issue: 2
• Pages: 633-639
• Publication date: 2010-03-01
• DOI: 10.1007/s00253-009-2305-0
• EISSN: 1432-0614
• ISSN: 0175-7598
• Language: English
• Keywords: Escherichia coli; Escherichia coli Proteins; Lepidoptera; Recombinant Proteins; HSP40 Heat-Shock Proteins; Genes, Reporter; HSP70 Heat-Shock Proteins; Larva; Protein Folding; Biotechnology; Green Fluorescent Proteins; Animals